Indiana University Bloomington
Flow Cytometry Core Facility

Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II 5-laser, 14-color sorter, the SH800 (New) 2-laser, 6-color user-run cell sorter, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a 4-laser, 14-color analyzer with HTS option, the MACSQuant VYB 3-laser, 8-color analyzer with 96-well plate option, the FACSCalibur (Retired), a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. Services include technician assisted sorting, as well as training for analyzers, SH800 and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and has a copy of this software as well as a site license for use outside of the facility. For more information and for any questions, please contact the facility.


July 20, 2020 - Email sent with the following topics: reminder that anyone interested in using the facility after the restart needs to schedule a Zoom meeting with Christiane, facility intro/safety training available over Zoom, facility-run analysis available, moveable clear partitions available to help separate instruments and researchers.

June 15, 2020 - Next phase for facility reopening starts Tuesday June 23, 2020 - Phase 2 information

June 5, 2020 - Email with updates and reminders was sent to the listserv with the following topics: NIEHS certificate (see May 29 announcement), paper towels on door handles, FlowJo dongles , 2 full weekdays'  notice for appointments, Restart Zooms               

May 29, 2020 - Researchers using the facility must comply with the facility restart plan (below), AND also the Biology Research Restart Policy and the College Research Restart Plan. Everyone using the facility must complete the NIEHS COVID-19 Response training and send the core manager (and anyone else who requires it) your certificate of completion          

May 28, 2020 - Information about Facility Appointments,  Protocols, and Q&A from the Zooms 

May 27, 2020 - Facility Initial Reopening Plan update 

May 21, 2020 - Facility Initial Reopening Plan - draft - facility reopening will occur based on local and university directives

April 7, 2020 - Updated facility policies for mitigation of COVID-19 spread and Appointment requests (also posted on the facility outer door)

March 19, 2020 - Facility policies to mitigate COVID-19 spread - posted on outer door of the facility

February 28 - March 4, 2020 - facility closed - manager at ABRF meeting

January 29 - February 4, 2020 - first training of facility users

January 9, 2020 - facility personnel receiving SH800 training

December 19 - January 1, 2020 - facility closed for the holidays

November 14 and 19, 2019 - self-service sorter install

November 5, 2019 - bioBUBBLE install for self-service sorter

October 1, 2019 - The Calibur is retired

Wednesday August 28, 2019 - FCS Express 7 workshop: 12-4pm, Jordan Hall 422

August 5-9, 2019 - Facility closed

June 10-16, 2019 - Facility closed

May 13, 14, 16, 17, 20, 2019 - Flow Cytometry Workshop

May 1-2, 2019 - Flow Facility hosting Bio-Rad S3e sorter demo. Presentation Wednesday May 1, 10-11am.



The "Mercury" was featured on two IU Twitter feeds:,

>> ABRF  article about acknowledgment of core facilities in publications:

>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at - go to "Grants and Funding"

>> We're on Facebook - find and like us at - you do not need to be a member of Facebook to view (just click on "close" in the bottom righthand corner if a box pops up)


Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (DGRC) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Roger Innes, Ph.D. (Biology) - Chair
Thom Kaufman, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)
Heather O'Hagan, Ph.D. (Med Sci)
Diego Ogando, Ph.D (Optometry)
Jonathan Schlebach, Ph.D (Chemistry)