Indiana University Bloomington
Flow Cytometry Core Facility

Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II 5-laser, 14-color sorter, the SH800 (New) 2-laser, 6-color user-run cell sorter, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a 4-laser, 14-color analyzer with HTS option, the MACSQuant VYB 3-laser, 8-color analyzer with 96-well plate option, the FACSCalibur (Retired), a four-color analyzer, as well as Z2 Coulter counter (currently out of order) technology used for determining cell concentration and size distribution. Services include technician assisted sorting, as well as training for analyzers, SH800 and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and has a copy of this software as well as a site license for use outside of the facility. For more information and for any questions, please contact the facility.

Announcements

June 28, 2022 - FluoroFinder seminar, 2pm - Register here

June 2-8, 2022 - Facility after-hours use only; CYTO 2022 meeting

June 1, 2022 - Curiox Laminar Wash Seminar, 1pm - Register here

May 30, 2022 - Memorial Day Holiday

April 18-22, 2022 - Facility after-hours use only

March 28-30, 2022 - Facility after-hours use only

January 17, 2022 - facility closed for Martin Luther King, Jr. holiday

December 20, 2021 - January 3, 2022 - facility closed for winter holidays

November 24-26, 2021 - facility closed for Thanksgiving holiday

November 15, 2021 - FCS Express seminar

November 4, 2021 - MACSQuant VYB seminar

October 11, 2021- SH800 seminar (This equipment was supported in part, with support from the Indiana Clinical and Translational Sciences Institute, funded in part by Grant Number UL1 TR002529 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award)

August 23, 2021 - SH800 in-hours training available

August 5, 2021 - masks are required of all facility researchers, regardless of vaccine status - Updated guidelines - PDF

July 9, 2021 - Updated facility guidelines - PDF

July 5, 2021 - Facility closed in observance of the Independence Day holiday 

May 31, 2021 - Facility closed for Memorial Day holiday

January 18, 2021 - Facility closed for Martin Luther King Jr, holiday

December 25, 2020 - January 3, 2021 - Facility closed for Christmas/New Year holidays and NO facility access during this time

November 25-27, 2020 - Facility closed for Thanksgiving holiday

July 20, 2020 - Email sent with the following topics: reminder that anyone interested in using the facility after the restart needs to schedule a Zoom meeting with Christiane, facility intro/safety training available over Zoom, facility-run analysis available, moveable clear partitions available to help separate instruments and researchers. Additional updates may be made that are not included in the Phase 2 document.

June 15, 2020 - Next phase for facility reopening starts Tuesday June 23, 2020 - Phase 2 information

June 5, 2020 - Email with updates and reminders was sent to the listserv with the following topics: NIEHS certificate (see May 29 announcement), paper towels on door handles, FlowJo dongles , 2 full weekdays'  notice for appointments, Restart Zooms               

May 29, 2020 - Researchers using the facility must comply with the facility restart plan (below), AND also the Biology Research Restart Policy and the College Research Restart Plan. Everyone using the facility must complete the NIEHS COVID-19 Response training and send the core manager (and anyone else who requires it) your certificate of completion          

May 28, 2020 - Information about Facility Appointments,  Protocols, and Q&A from the Zooms 

May 27, 2020 - Facility Initial Reopening Plan update 

May 21, 2020 - Facility Initial Reopening Plan - draft - facility reopening will occur based on local and university directives

April 7, 2020 - Updated facility policies for mitigation of COVID-19 spread and Appointment requests (also posted on the facility outer door)

March 19, 2020 - Facility policies to mitigate COVID-19 spread - posted on outer door of the facility

February 28 - March 4, 2020 - facility closed - manager at ABRF meeting

January 29 - February 4, 2020 - first training of facility users for SH800  

January 9, 2020 - facility personnel receiving SH800 training

 

 

The "Mercury" was featured on two IU Twitter feeds: https://twitter.com/IUBiology/status/878279482011918336, https://twitter.com/TuliLab/status/878267877509533696

>> ABRF  article about acknowledgment of core facilities in publications: https://abrf.org/authorship-guidelines

>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at https://www.indianactsi.org/ - go to "Grants and Funding"

>> We're on Twitter - find us at https://twitter.com/iubfccf - you do not need to be a member of Twitter to view

 

Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (DGRC) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Roger Innes, Ph.D. (Biology) - Chair
Thom Kaufman, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)
Heather O'Hagan, Ph.D. (Med Sci)
Diego Ogando, Ph.D (Optometry)
Jonathan Schlebach, Ph.D (Chemistry)