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Indiana University Bloomington
Flow Cytometry Core Facility

Data Analysis

FCS Express and FlowJo

FCS Express and FlowJo are both powerful off-line cytometry data analysis software programs.  Both programs can analyze all flow cytometry data files in FCS format, and are compatible with both Mac and Windows systems. 

FCS Express:

The facility has an internet site license for the FCS Express program. Any IU researcher may download the program and run it on their computer via an internet connection - no dongle is necessary. The cost per license is $234/registration annually and includes unlimited use of FCS Express. The license registration will renew yearly on July 1 with a valid IU lab account number. Please note that registration is not prorated.

If you would like to test FCS Express, first register for a De Novo account at: https://denovosoftware.com/register/ and then go here: https://denovosoftware.com/demo-overview/

If you are interested in purchasing a license, submit a request at: https://www.denovosoftware.com/RegisterForSiteLicense/?SignupCode=IUBFCCF - and email the facility with a valid account number.

The site license comes with yearly training which will be announced via the flow facility listserv. Webinars can be found at: webinars.

Please contact Christiane Hassel for more information.

Flow Jo:

We currently have two copies of the program available in dongle (USB) format that may be signed out. A copy of the FlowJo program can be downloaded at https://www.flowjo.com/solutions/flowjo/downloads. Both traveling dongles can be used on Apple and Windows systems. 

FlowJo tutorials and videos can be found on the links page of the website.

Availability of traveling and workstation FlowJo copies can be checked on the calendar page of the website (calendar is read-only).

Reservations for the FlowJo dongle must be made at least one full business day in advance.  

The maximum sign-out time for the traveling dongle is: 24 hours (or through the weekend if requested on a Friday).

Please contact Christiane Hassel to schedule an appointment for use of a FlowJo dongle and for more information. 

A very important note:  If the dongle is damaged or lost, a cost recovery fee will be assessed. The current cost to replace a damaged dongle is $230. The current cost to replace a lost dongle is $2,025.

FlowJo legend:
(Red) Facility schedule (applies to all instrumentation and software)
(Olive) FlowJo traveling dongle